Induction of APOBEC3G ubiquitination and degradation by an HIV-1 Vif-Cul5-SCF complex.

Induction of Ubiquitination and Degradation of APOBEC3G by HIV-1 Vif-Cul5-SCF

Phosphorylation of a novel SOCS-box regulates assembly of the HIV-1 Vif-Cul5 complex that promotes APOBEC3G degradation.

HIV-1 Vif (viral infectivity factor) protein overcomes the antiviral activity of the DNA deaminase APOBEC3G by targeting it for proteasomal degradation. We report here that Vif targets APOBEC3G for degradation by forming an SCF-like E3 ubiquitin ligase containing Cullin 5 and Elongins B and C (Cul5-EloB-EloC) through a novel SOCS (suppressor of cytokine signaling)-box that binds EloC. Vif bindi...

A zinc-binding region in Vif binds Cul5 and determines cullin selection.

Human immunodeficiency virus-1 (HIV-1) Vif overcomes the anti-viral activity of APOBEC3G by targeting it for ubiquitination via a Cullin 5-ElonginB-ElonginC (Cul5-EloBC) E3 ligase. Vif associates with Cul5-EloBC through a BC-box motif that binds EloC, but the mechanism by which Vif selectively recruits Cul5 is poorly understood. Here we report that a region of Vif (residues 100-142) upstream of...

Vif hijacks CBF-b to degrade APOBEC3G and promote HIV-1 infection

Restriction factors, such as the retroviral complementary DNA deaminase APOBEC3G, are cellular proteins that dominantly block virus replication. TheAIDSvirus, human immunodeficiency virus type 1 (HIV-1), produces the accessory factor Vif, which counteracts the host’s antiviral defence by hijacking a ubiquitin ligase complex, containing CUL5, ELOC, ELOB and a RING-box protein, and targeting APOB...

Associations between HIV Susceptibility and Mutations in the Vif-associated APOBEC3G Proteasomal Complex

The purpose of this experiment was to determine whether a host’s single nucleotide polymorphisms (SNPs) in the Vifassociated APOBEC3G Proteasomal Complex influence his/her innate immunity to Human Immunodeficiency Virus-1. The experimenters compared host DNA at specific sites on each patient’s genome (SNPs) and the patient’s HIV1 disease progression. They extracted DNA from peripheral blood mon...